Speaker
Description
DNA tracers have been applied in groundwater systems in order to track flow paths and travel times, and to identify hydraulic connections. In this study, we aim to develop a blueprint of the application of DNA tracers to determine hydraulic conductivity, effective porosity, and dispersion. Also, we aim to minimize the uncertainty in estimating the parameters. Two well-controlled laboratory built, ~130 x 70 x 40 cm and ~100 x 58 x 30 cm, respectively, Aquifer tanks were used under steady-state flow conditions. Under forced gradient condition, monodispersed, silica-coated double-stranded DNA tracer particles were injected and collected down-gradient. Different injection and sampling experiments were carried out: various well configurations, sampling depths, injection intervals and so forth. Water samples collected were analyzed on their DNA tracer concentration using a qPCR machine. Finally, the resulting breakthrough curves were analyzed to arrive at hydraulic properties, using groundwater flow and contaminant transport modeling (MT3DMS).
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