19–22 May 2026
Europe/Paris timezone

Selective Plane Imaging Microscopy (SPIM) for 3D imaging of mixing and bacteria colonization in porous media.

21 May 2026, 15:35
1h 30m
Poster Presentation (MS02) Environmental Porous Media: Water, Agriculture, and Remediation Poster

Speaker

Valentine Rollot (Postdoc)

Description

This study aims to investigate the behavior of microbial communities under flow conditions in porous, non-homogeneous 3D environments. Indeed, the majority of microbial communities are known to develop in microstructures, such as in soil or lung pores, and are subject to large variations in the concentrations of dissolved elements (O₂, nutrients, etc.). The objective of this study is then to determine how 3D chaotic flow controls microbial motility and colonization.

This work is conducted through the development of a new method for 3D imaging using laser-induced fluorescence and optical index adjustment. Columns of hydrogel beads mimic the porous 3D environment where bacterial strains are injected in a continuous and steady-state flow. Behavior of Pseudomonas putida KT2440 strains are compared to passive (non-swimming) fluorescent beads and solute.

Country france
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Author

Valentine Rollot (Postdoc)

Co-authors

Despoina Anastasopoulou (Institut de Mécanique des Fluides de Toulouse, UMR 5502 CNRS Institut National Polytechnique de Toulouse, 31400 Toulouse, France and Géosciences Rennes, UMR 6118 CNRS Université de Rennes, 35000 Rennes, France) Marc Lamblin Manuel Maeritz (University of Rennes 1) Joris Heyman (CNRS) Tanguy Le Borgne (Université de Rennes)

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