22–25 May 2023
Europe/London timezone

Microfluidic study of hydrogen conversion by Archea in porous media. A pore-scale investigation of gas conversion and controlling parameters under dynamic conditions.

23 May 2023, 16:10
1h 30m
Poster Presentation (MS05) Biochemical processes and biofilms in porous media Poster

Speaker

Patrick Jasek

Description

Patrick Jasek 1, Hannes Koneggerv2,Saeid Sadeghnejad 3, Frieder Enzmann 3,Andreas Loibner 2, Holger Ott 3
1 Department Petroleum Engineering, Montanuniversität Leoben, 8700 Leoben, Austria
2 Institut für Umweltbiotechnologie, Universität für Bodenkultur Wien,3430 Tulln an der Donau, Austria
3 Institut für Geowissenschaften, Johannes Gutenberg-Universität Mainz, D-55128 Mainz

The necessity to scale energy storage capacities in Europe is vital for expanding renewable energy production and avoiding shortcomings in energy supply. In decarbonizing energy systems, hydrogen, in its diverse colors, will play a crucial role as an energy carrier. Storing hydrogen in the subsurface comes with various uncertainties. Independent investigations of underground hydrogen storage revealed that hydrogen is utilized as substrate by Archean cultures in the reservoir leading to environment-controlled reactions.
This study investigates site-extracted and brine-suspended cultures in a microfluidic setup. Biomass accumulation in pore space under saturated and anaerobic flow conditions has shown that bacteria significantly change the hydraulic properties of porous media, altering both porosity and permeability. Using time-lapse imaging, different modes of bacterial accumulation and preferential channel formation could be observed as a function of the injection velocity. The extracted time series was used in numerical Stokes–Brinkmann flow simulations to estimate intrinsic biomass permeability, velocities, and stresses. The simulation results were validated with experimental data and revealed an average intra-biomass permeability of 100±30 mD.
Further analyses concerning the advective nutrient supply potential were conducted to estimate the controlling parameters like the Peclet number. The selected velocity impacts the average biomass cluster size and, herewith, also, the nutrient distribution within the biomass. Furthermore, biomass activity in the presence of a nutrient gas blend of carbon dioxide and hydrogen was investigated in a dynamic flow experiment at elevated pressure and temperature.
The novel setup allowed us to continuously monitor biomass growth, related system responses, and compositional gas changes. Conclusively, an advective nutrient supply in biomass is considered more efficient than a purely diffusive one and might lead to higher metabolic activity and substrate conversion rates, which is particularly interesting for engineered subsurface applications.

Participation In-Person
Country Österreich
Energy Transition Focused Abstracts This abstract is related to Energy Transition
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Primary authors

Patrick Jasek Holger Ott (Montanuniversität Leoben)

Co-authors

Hannes Konegger Saeid Sadeghnejad Prof. Loibner Andreas Frieder Enzmann

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