22–25 May 2023
Europe/London timezone

In situ imaging of bacteria transport and attachment in geologic materials using positron emission tomography

23 May 2023, 14:45
15m
Oral Presentation (MS05) Biochemical processes and biofilms in porous media MS05

Speaker

Mrs Vy Le (University of Wisconsin-Madison)

Description

Contamination of groundwater by Escherichia coli (E. coli) bacteria has been a persistent and growing risk to globally strained groundwater resources. Prevention, mitigation, and regulation of contaminants requires a fundamental understanding of the mechanisms of transport and attachment of E. coli in complex geological materials under hydrogeologic conditions. This work demonstrates the first experimental quantification of dynamic bacteria transport and attachment distributions in geologic materials using 3D medical imaging. The approach relies on radiolabeling E. coli bacteria with positron-emitting radioisotopes and then using positron emission tomography (PET) to monitor bacterial distribution and transport in heterogeneous sand packed column experiments. The results of this study indicate that bacteria attachment coefficient distributions are described by gamma probability density functions. As expected, these functions shift to higher attachment coefficients with decreasing grain size and decreased sediment sorting. Results from these radiolabeling and imaging techniques provide a transformational approach to directly measure and understand dynamic bacteria attachment and detachment behavior in realistic geologic systems.

Participation In-Person
Country United States
MDPI Energies Student Poster Award No, do not submit my presenation for the student posters award.
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Primary authors

Prof. Christopher Zahasky (University of Wisconsin-Madison) Mrs Vy Le (University of Wisconsin-Madison)

Presentation materials

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